Examples of Strain development programmes
Below is a selection of microorganisms and their respective natural products for which Novacta scientists successfully optimised production.
- Actinoplanes sp. - lantibiotics
- Streptomyces citricolor - aristeromycin
- Streptomyces sp. - carbapenems
- Streptomyces sp. - milbermycin endectocide
- Amycolatopsis orientalis - vancomycin
- Sordaria araneosa -sordarin titre improvement / biosynthetic mutants
-
Phoma sp - squalestatin
Example
- Conventional mutagenesis on spores (UV, MNNG)
- Mutagenized colonies screened in 96-well microtitre plates (biosynthetic mutants) and miniaturised culture vessels (titre improvement)
- Plates loaded directly onto HPLC, <2 minute mini column
- Mutants obtained:
- Block in diterpene (no product)
- Aglycone
- Sugar alterations - 100kg batches of modified product
- Improved titre to commercial production scale
