The Nycodenz gradient solutions for the preparation of essentially iso-osmotic Nycodenz® gradients has been devised and these can be prepared usinganiso-osmotic solution of Nycodenz®which contains:
27.6% (w/v) Nycodenz® (density = 1.15 g/ml) made up in
buffered medium.
This solution may be diluted to desired
concentration by using a buffered diluent containing either
sucrose or NaCl as osmotic balancer.
The composition of these diluents are as follows:
- 0.75 g NaCl or 7.45 g sucrose dissolved in 100 ml 5 mmol/I TrisHCI (pH 7.5) containing 3 mmol/l KCI and 0.3 mmol/I
CaNa2EDTA.
The relationship between density and refractive index (n) can NaCldiluent Sucrose diluent
Density = 3.287 n - 3.383 Density = 3.410 n - 3.555iting